Purpose: We intend to provide the basic data for the creation of a working environment and improvement of work methods to prevent burnout of female care workers by identifying the factors affecting their work burnout of female care workers. Methods: We used logistic regression analysis to analyze the relations of 1,159 people classified as care workers using the 6th Working Environment Survey. Results: There was a significant difference in the rate of experiencing burnout depending on the general, working environment, and health-related characteristics of female care workers. In general characteristics, workers in their 50s and older experienced more work burnout when the average working hours per week were over 40 hours. In work environment characteristics, the work of lifting or moving people and the work exhaustion of workers dealing with angry people caused more work burnout. In health-related characteristics, it was found that workers who suffered from work stress and experienced headache and fatigue experienced more work burnout. Conclusion: It is necessary to improve social awareness of care services and strengthen legal protective measures for care workers. It is also essential to prepare a detailed work manual suitable for care workers and to train coping. In addition, customized education and various programs for the physical and formal health of female care workers should be activated.