Purpose : The purpose of this study is to investigate the effect of general hospital nurses' sleep disturbance and organizational commitment on job satisfaction. Methods : The data were collected using structured questionnaires from 180 nurses working at 3 general hospitals located in Seoul and Gyeonggi. The collected data were analyzed using the mean, standard deviation, t-test, ANOVA, Pearson's correlation, and stepwise multiple regression analysis using SPSS 21.0. Results : As a result, sleep disturbance was average of 3.02 out of 5, organizational commitment was average of 2.76 out of 5, and job satisfaction was 2.87 out of 5. Sleep disturbance, organizational commitment and job satisfaction were statistically correlated. Organizational commitment (β=.59, p<.001) and total length of clinical career (β=.12, p=.031) had a significant effect on job satisfaction. This factors explained 44.7% of job satisfaction (F=21.67, p<.001). Conclusion : Based on the results of this study, in order to increase the job satisfaction of hospital nurses, it is necessary to systematically improve the intervention method to promote sleep and an appropriate compensation system for organizational commitment.